Login processing...

Trial ends in Request Full Access Tell Your Colleague About Jove
JoVE Journal
Bioengineering

A subscription to JoVE is required to view this content.
You will only be able to see the first 20 seconds.

Measuring Global Cellular Matrix Metalloproteinase and Metabolic Activity in 3D Hydrogels
 

Measuring Global Cellular Matrix Metalloproteinase and Metabolic Activity in 3D Hydrogels

Article doi: 10.3791/59123
January 22nd, 2019 Usage Statistics

Summary January 22nd, 2019

Here, a protocol is presented for encapsulating and culturing cells in poly(ethylene glycol) (PEG) hydrogels functionalized with a fluorogenic matrix metalloproteinase (MMP)-degradable peptide. Cellular MMP and metabolic activity are measured directly from the hydrogel cultures using a standard microplate reader.

Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
simple hit counter