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Nを用いたシアル酸の代謝独自-アシル-Mannosamines を変更
JoVE Journal
Bioengineering
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JoVE Journal Bioengineering
Metabolic Glycoengineering of Sialic Acid Using N-acyl-modified Mannosamines
DOI:

12:06 min

November 25, 2017

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Chapters

  • 00:05Title
  • 01:02Synthesis of ManNProp and Related N-acyl-modefied Mannosamines
  • 02:26Cell Culture and Cell Lysis for HPLC Analysis
  • 04:28Separation of the Membrane Fraction and Acid Hydrolysis
  • 05:27Fluorescent Labeling, HPLC, and Mass Spetrometry Analysis
  • 06:55Western Blot Analysis of Polysialic Acid in Kelly Neuroblastoma Cells
  • 09:00Results: Modulating Expression of Sialic Acid on Cell Surfaces Using N-Side Chain Modified Mannosamines
  • 10:40Conclusion

Summary

Automatic Translation

シアル酸は、典型的な単糖単位の複合糖質は、です。分子と細胞の相互作用の茄多になっています。代謝独自のN- acetylmannosamine 誘導体を用いた細胞表面のシアル酸の発現を変更する方法をご紹介します。

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