Real-time Iontophoresis with Tetramethylammonium to Quantify Volume Fraction and Tortuosity of Brain Extracellular Space

John Odackal; Robert Colbourn; Namrita Jain Odackal; Lian Tao; Charles Nicholson; Sabina Hrabetova

doi:10.3791/55755

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Iontoforesi in tempo reale con tetrametilammonio per quantificare la frazione del volume e la tortuosità dello spazio extracellulare del cervello

Real-time Iontophoresis with Tetramethylammonium to Quantify Volume Fraction and Tortuosity of Brain Extracellular Space

JoVE Journal
Neuroscience

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JoVE Journal Neuroscience

Real-time Iontophoresis with Tetramethylammonium to Quantify Volume Fraction and Tortuosity of Brain Extracellular Space

Iontoforesi in tempo reale con tetrametilammonio per quantificare la frazione del volume e la tortuosità dello spazio extracellulare del cervello

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11,819 Views

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10:45 min

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July 24, 2017

DOI:

10.3791/55755-v

DOI:

10.3791/55755-v

•

10:45 min

July 24, 2017

•

2 Views

John Odackal*¹, Robert Colbourn*^2,3, Namrita Jain Odackal⁴, Lian Tao⁵, Charles Nicholson⁵, Sabina Hrabetova²

¹Department of Medicine,University of Virginia, ²Department of Cell Biology,SUNY Downstate Medical Center, ³Neural and Behavioral Science Graduate Program,SUNY Downstate Medical Center, ⁴Division of Neonatology,University of Virginia, ⁵Department of Neuroscience and Physiology,New York University School of Medicine

Transcript

Summary

Questo protocollo descrive la iontoforesi in tempo reale, un metodo che misura i parametri fisici dello spazio extracellulare (ECS) dei cervelli viventi. La diffusione di una molecola inerte rilasciata nell'ECS viene usata per calcolare la frazione del volume ECS e la tortuosità. È ideale per studiare cambiamenti reversibili acuti al cervello ECS.