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脊髓神经元从新生小鼠中分离和培养

JoVE Journal
Neuroscience

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JoVE Journal Neuroscience

Spinal Cord Neurons Isolation and Culture from Neonatal Mice

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脊髓神经元从新生小鼠中分离和培养

DOI:

10.3791/55856-v

•

07:49 min

•

July 11, 2017

•

Mohamed Eldeiry, Katsuhiro Yamanaka, T. Brett Reece, Muhammad Aftab

¹Department of Surgery, Division of Cardiothoracic Surgery,University of Colorado Anschutz Medical Campus

Chapters

00:05Title
00:58Harvesting Spinal Cords
02:27Isolating Neurons
05:53Results: Immunofluorescent Staining of Neurons Using Neuronal Cytoskeletal Marker – MAP2
06:47Conclusion

Summary

Automatic Translation

这项研究提出了一种从WT新生小鼠中分离神经元的技术。它需要从新生小鼠中仔细地解剖脊髓，然后通过机械和酶切割将脊髓组织中的神经元分离出来。

Tags

Spinal Cord Neurons Neuron Isolation Neuron Culture Neonatal Mice Density Gradient Trituration Neuron Extraction Neurobiology Experiments

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