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Analysis of the Gap Junction-dependent Transfer of miRNA with 3D-FRAP Microscopy
JoVE Journal
Biology
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JoVE Journal Biology
Analysis of the Gap Junction-dependent Transfer of miRNA with 3D-FRAP Microscopy
DOI:

06:26 min

June 19, 2017

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Chapters

  • 00:05Title
  • 00:38Transfection of Cardiomyocytes with Fluorescently Labeled miRNA
  • 01:56Applying 3D-FRAP Microscopy and Data Analysis
  • 04:08Results: 3D FRAP Analysis of Gap Junction-dependent Transfer of miRNA
  • 05:42Conclusion

Summary

Automatic Translation

Here, we describe the application of three-dimensional fluorescence recovery after photobleaching (3D-FRAP) for the analysis of the gap junction-dependent shuttling of miRNA. In contrast to commonly applied methods, 3D-FRAP allows for the quantification of the intercellular transfer of small RNAs in real time, with high spatio-temporal resolution.

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