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用3D-FRAP显微镜分析miRNA的间隙连接依赖性转移
JoVE Journal
Biology
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JoVE Journal Biology
Analysis of the Gap Junction-dependent Transfer of miRNA with 3D-FRAP Microscopy
DOI:

06:26 min

June 19, 2017

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Chapters

  • 00:05Title
  • 00:38Transfection of Cardiomyocytes with Fluorescently Labeled miRNA
  • 01:56Applying 3D-FRAP Microscopy and Data Analysis
  • 04:08Results: 3D FRAP Analysis of Gap Junction-dependent Transfer of miRNA
  • 05:42Conclusion

Summary

Automatic Translation

在这里,我们描述了光漂白后三维荧光恢复(3D-FRAP)的应用,用于分析miRNA的间隙连接依赖穿梭。与通常应用的方法相比,3D-FRAP允许实时定量小RNA的细胞间转移,具有高的时空分辨率。

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