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用3D-FRAP显微镜分析miRNA的间隙连接依赖性转移

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Biology

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JoVE Journal Biology

Analysis of the Gap Junction-dependent Transfer of miRNA with 3D-FRAP Microscopy

Please note that all translations are automatically generated. Click here for the English version.

用3D-FRAP显微镜分析miRNA的间隙连接依赖性转移

DOI:

10.3791/55870-v

•

06:26 min

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June 19, 2017

•

Heiko Lemcke^2,3, Natalia Voronina^2,3, Gustav Steinhoff^2,3, Robert David^2,3

¹Reference and Translation Center for Cardiac Stem Cell Therapy (RTC), ²Department of Cardiac Surgery,University of Rostock, ³Department of Life, Light and Matter of the Interdisciplinary Faculty,University of Rostock

Chapters

00:05Title
00:38Transfection of Cardiomyocytes with Fluorescently Labeled miRNA
01:56Applying 3D-FRAP Microscopy and Data Analysis
04:08Results: 3D FRAP Analysis of Gap Junction-dependent Transfer of miRNA
05:42Conclusion

Summary

Automatic Translation

在这里，我们描述了光漂白后三维荧光恢复（3D-FRAP）的应用，用于分析miRNA的间隙连接依赖穿梭。与通常应用的方法相比，3D-FRAP允许实时定量小RNA的细胞间转移，具有高的时空分辨率。

Tags

Gap Junction Micro RNA 3D-FRAP Microscopy Cardiomyocytes Cell Culture Electroporation Confocal Microscopy Fluorescence Recovery After Photobleaching (FRAP)

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