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荧光介导的断层扫描对巨噬细胞相关小鼠肠道炎症的检测和定量

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JoVE Journal Medicine

Fluorescence-mediated Tomography for the Detection and Quantification of Macrophage-related Murine Intestinal Inflammation

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荧光介导的断层扫描对巨噬细胞相关小鼠肠道炎症的检测和定量

DOI:

10.3791/55942-v

•

07:05 min

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December 15, 2017

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Tobias M. Nowacki, Dominik Bettenworth, Markus Brückner, Friederike Cordes, Frank Lenze, Anne Becker, Moritz Wildgruber, Michel Eisenblätter

¹Department of Medicine B,University Hospital Münster, ²Translational Research Imaging Center, Department of Clinical Radiology,University Hospital Münster

Chapters

00:05Title
00:51Fluorescence-mediated Tomography (FMT)
03:33Data Reconstruction and Interpretation
04:47Results: Representative Fluorescence-mediated Tomography
06:17Conclusion

Summary

Automatic Translation

目标特定的探针代表了一种用于分析分子机制的创新工具, 例如各种疾病的蛋白质表达 (例如,炎症、感染和肿瘤发生)。在这项研究中, 我们描述了一个定量的三维层析成像评估肠道巨噬细胞浸润的小鼠结肠炎模型使用 F4/80-specific 荧光介导的断层扫描。

Tags

Fluorescence-mediated Tomography Macrophage Intestinal Inflammation Inflammatory Bowel Disease In Vivo Imaging Dextran Sodium Sulfate Antibody Fluorescence-mediated Tomography Device Tracer Anesthetized Mouse Fluorescence Reflectance Image

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