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腺相关病毒对大鼠的静脉管理方法和技巧及中枢神经系统转导的评价
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Neuroscience
Methods and Tips for Intravenous Administration of Adeno-associated Virus to Rats and Evaluation of Central Nervous System Transduction
Please note that all translations are automatically generated.
Click here for the English version.
腺相关病毒对大鼠的静脉管理方法和技巧及中枢神经系统转导的评价
DOI:
10.3791/55994-v
•
08:11 min
•
August 25, 2017
•
Mychal S. Grames
,
Kasey L. Jackson
,
Robert D. Dayton
,
John A. Stanford
,
Ronald L. Klein
1
Department of Pharmacology, Toxicology, and Neuroscience
,
Louisiana State University Health Sciences Center
,
2
Department of Molecular & Integrative Physiology
,
University of Kansas Medical Center
Chapters
00:05
Title
00:58
Preparation for Injection
02:47
Vector Injection
04:55
Quantification of GFP Expression in the Cerebellum
06:48
Rapid Quantification of the GFP-positive Fluorescent Area in the Cerebellum After Intravenous AAV Administration to the Rat
07:30
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
方法为广泛的中枢神经系统基因交付在大鼠被盖。在这个例子中, 目的是模拟一种疾病, 影响整个脊髓。广泛的转导可以用来提供一个治疗蛋白的中枢神经系统从一个 one-time, 外围管理。
Tags
Intravenous Administration
Adeno-associated Virus
Rats
Central Nervous System Transduction
Gene Transfer
Amyotrophic Lateral Sclerosis
Spinal Cord
Anesthesia
Tail Vein Injection
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