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Metoder og Tips til intravenøs indgift af Adeno-associeret Virus at rotter og evaluering af centralnervesystemet transduktion
JoVE Journal
Neuroscience
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JoVE Journal Neuroscience
Methods and Tips for Intravenous Administration of Adeno-associated Virus to Rats and Evaluation of Central Nervous System Transduction
DOI:

08:11 min

August 25, 2017

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Chapters

  • 00:05Title
  • 00:58Preparation for Injection
  • 02:47Vector Injection
  • 04:55Quantification of GFP Expression in the Cerebellum
  • 06:48Rapid Quantification of the GFP-positive Fluorescent Area in the Cerebellum After Intravenous AAV Administration to the Rat
  • 07:30Conclusion

Summary

Automatic Translation

Metoder til en omfattende centralnervesystemet gen levering i rotten er dækket. I dette eksempel er formålet at efterligne en sygdom, der påvirker hele rygmarven. Den udbredte transduktion kan bruges til at levere en terapeutisk protein til CNS fra en engangs, perifere administration.

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