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核密度分析的天然染色质沉淀测序文库的生成
JoVE Journal
Genetics
This content is Free Access.
JoVE Journal Genetics
Generation of Native Chromatin Immunoprecipitation Sequencing Libraries for Nucleosome Density Analysis
DOI:

10:05 min

December 12, 2017

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Chapters

  • 00:05Title
  • 00:51Cell Lysis and Chromatin Digestion for Nucleosome Density ChIP-se
  • 03:21Immunprecipitation Reaction for Nucleosome Density ChIP-seq
  • 04:33Washes and Elution for Nucleosome Density ChIP-seq
  • 07:31Results: Evaluation of Nucleosome Density ChIP-seq Libraries
  • 08:50Conclusion

Summary

Automatic Translation

我们提出了一个改良的本地染色质沉淀测序 (芯片-seq) 的方法, 以生成序列数据集适合核密度芯片-seq 分析框架集成 micrococcal 核酸 (MNase) 可及性组蛋白修饰测量。

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