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传染性噬菌体大肠杆菌-合成基于无细胞表达系统
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Bioengineering
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JoVE Journal Bioengineering
Synthesis of Infectious Bacteriophages in an E. coli-based Cell-free Expression System
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传染性噬菌体大肠杆菌-合成基于无细胞表达系统

DOI:
10.3791/56144-v

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11:33 min

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August 17, 2017

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, , , ,
1School of Physics and Astronomy,University of Minnesota, 2Department of Diagnostic and Biological Sciences and Institute for Molecular Virology,University of Minnesota

Chapters

  • 00:05Title
  • 00:37Phage Amplification
  • 02:15Purification of Phage
  • 05:39Double-stranded Genome DNA Extraction
  • 08:19Cell-free Phage Reaction and Phage Titer Experiment
  • 09:35Results: Synthesis and Quantification of Phage T7 in Varying Molecular-crowding Environments
  • 10:46Conclusion

Summary

Automatic Translation

Automatic Translation

新一代的无细胞的转录翻译平台被设计在生化系统体外基因电路执行构建。在这篇文章中，我们描述如何从其基因组，使用所有的大肠杆菌细胞免费 TXTL 系统合成噬菌体 MS2、 ΦΧ174 和 T7，等。

Tags

Bacteriophage SynthesisE. Coli Cell-free Expression SystemPhage PlaquesPhage InfectionDNaseSucrose GradientUltracentrifugationPhage Band Extraction

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