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人脑脊液 Endopeptidomic 分析的样品制备
JoVE Journal
Neuroscience
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JoVE Journal Neuroscience
Sample Preparation for Endopeptidomic Analysis in Human Cerebrospinal Fluid
DOI:

10:23 min

December 04, 2017

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Chapters

  • 00:05Title
  • 01:12Pre-treatment of Bulk Volume CSF without Quantification
  • 02:24Pre-treatment of Small Volume CSF Samples with Isobaric Labeling
  • 04:00MWCO Filtration, Desalting, and Sample Clean-up by Solid Phase Extraction
  • 06:00Offline High-pH Reverse Phase HPLC Sample Fractionation
  • 07:09LC-MS Analysis
  • 07:47Results: Peptide Identification After LC-MS Analysis
  • 09:13Conclusion

Summary

Automatic Translation

提出了一种人脑脊液内源性多肽的质谱分析方法。通过采用分子量切断过滤、色谱预分馏、质谱分析以及随后的多肽识别策略组合, 就可以将已知的脑脊液 peptidome 扩大近十倍以前的研究。

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