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Neuroscience
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人脑脊液 Endopeptidomic 分析的样品制备
JoVE Journal
Neuroscience
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JoVE Journal
Neuroscience
Sample Preparation for Endopeptidomic Analysis in Human Cerebrospinal Fluid
Please note that all translations are automatically generated.
Click here for the English version.
人脑脊液 Endopeptidomic 分析的样品制备
DOI:
10.3791/56244-v
•
10:23 min
•
December 04, 2017
•
Karl T. Hansson
,
Tobias Skillbäck
2
,
Elin Pernevik
,
Jessica Holmén-Larsson
,
Gunnar Brinkmalm
,
Kaj Blennow
2
,
Henrik Zetterberg
2,3
,
Johan Gobom
2
1
Inst. of Neuroscience and Physiology, Dept. of Psychiatry and Neurochemistry
,
Sahlgrenska Academy at University of Gothenburg
,
2
Clinical Neurochemistry Laboratory
,
Sahlgrenska University Hospital
,
3
Department of Molecular Neuroscience
,
UCL Institute of Neurology
Chapters
00:05
Title
01:12
Pre-treatment of Bulk Volume CSF without Quantification
02:24
Pre-treatment of Small Volume CSF Samples with Isobaric Labeling
04:00
MWCO Filtration, Desalting, and Sample Clean-up by Solid Phase Extraction
06:00
Offline High-pH Reverse Phase HPLC Sample Fractionation
07:09
LC-MS Analysis
07:47
Results: Peptide Identification After LC-MS Analysis
09:13
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
提出了一种人脑脊液内源性多肽的质谱分析方法。通过采用分子量切断过滤、色谱预分馏、质谱分析以及随后的多肽识别策略组合, 就可以将已知的脑脊液 peptidome 扩大近十倍以前的研究。
Tags
Endopeptidomic Analysis
Cerebrospinal Fluid
Sample Preparation
LC-MS
Peptide Pre-fractionation
Endogenous Peptides
Biomarkers
Neurodegenerative Disorders
MWCO Filtration
High-pH Reverse Phase HPLC
Mass Spectrometry
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