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Developmental Biology
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人胚胎或诱导多能干细胞对视网膜色素上皮细胞的快速定向分化
JoVE Journal
Developmental Biology
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Developmental Biology
Rapid, Directed Differentiation of Retinal Pigment Epithelial Cells from Human Embryonic or Induced Pluripotent Stem Cells
Please note that all translations are automatically generated.
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人胚胎或诱导多能干细胞对视网膜色素上皮细胞的快速定向分化
DOI:
10.3791/56274-v
•
10:48 min
•
October 30, 2017
•
Leah P. Foltz
,
Dennis O. Clegg
1
Center for Stem Cell Biology and Engineering
,
University of California, Santa Barbara
Chapters
00:05
Title
01:02
Day 0: Day of Pluripotent Stem Cell Passage for Differentiation
04:43
Day of Enrichment to Passage 0 of Retinal Piment Epithelial Cells
08:37
Results: Representative Morphology and Confluence of Maturing RPE
09:48
Conclusion
Summary
Automatic Translation
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Automatic Translation
该协议描述了如何产生多能干细胞的视网膜色素上皮细胞 (RPE)。该方法结合生长因子和小分子, 将干细胞分化为未成熟的 rpe, 在十四天后成熟, 功能性视网膜色素上皮细胞三月后。
Tags
Retinal Pigment Epithelial Cells
Human Embryonic Stem Cells
Induced Pluripotent Stem Cells
Retinal Differentiation
Directed Differentiation
Stem Cell Biology
Eye Research
RPE
Retinal Development
Stem Cell Disease Model
Growth Factors
Small Molecules
Extracellular Matrix
Hydrogel
EDTA
Cell Passaging
Cell Removal
Undifferentiated Stem Cells
Efficiency
Enrichment
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