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应用多电极阵列评价内分泌干扰物对脊椎动物神经网络功能发育的影响
JoVE Journal
Developmental Biology
This content is Free Access.
JoVE Journal Developmental Biology
Assessment of the Effects of Endocrine Disrupting Compounds on the Development of Vertebrate Neural Network Function Using Multi-electrode Arrays
DOI:

08:28 min

April 26, 2018

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Chapters

  • 00:05Title
  • 01:03Preparation of the Multi-electrode Array for Embryonic Neuron Culture
  • 02:11Chick Embryonic Neuron Culture
  • 05:00Recording Neuronal Network Activity
  • 07:07Results: The Effect of BPA on Synchrony Development in Chick Embryo Neuronal Cultures
  • 07:32Conclusion

Summary

Automatic Translation

暴露在环境中的毒素会对发育中的胚胎产生剧烈影响。内分泌干扰的化学物质, 如 bisphenols, 对神经系统有负面影响。在这里, 我们描述了一个协议使用体外脊椎动物 (小鸡胚) 神经元网络模型来研究毒素暴露对早期胚胎的功能影响。

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