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基于芯片的三维细胞培养,在灌注微生物反应器
JoVE Journal
Biology
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JoVE Journal
Biology
Chip-based Three-dimensional Cell Culture in Perfused Micro-bioreactors
Please note that all translations are automatically generated.
Click here for the English version.
基于芯片的三维细胞培养,在灌注微生物反应器
DOI:
10.3791/564-v
•
12:39 min
•
May 21, 2008
•
Eric Gottwald
,
Brigitte Lahni
,
David Thiele
,
Stefan Giselbrecht
,
Alexander Welle
,
Karl-Friedrich Weibezahn
1
Institute for Biological Interfaces
,
Forschungszentrum Karlsruhe
Chapters
00:08
Introduction
00:27
CellChip preparation: Hydrophilization, coating, incubation
03:41
Bioreactor Setup
07:43
Incubation Setup
08:58
Analysis of organotypic functions
Summary
Automatic Translation
English (Original)
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Automatic Translation
我们描述了一个在微型生物反应器培养细胞的三维芯片为基础的平台。一个芯片上可以容纳10百万。无菌,封闭的循环回路中流体流动,氧张力等方面的准确界定条件下,可以培养细胞。
Tags
Chip-based
Three-dimensional Cell Culture
Perfused Micro-bioreactors
Chip
Non-biodegradable Polymers
Bio-degradable Polymers
Dimensions
Cubic Micro-containers
Round Recesses
Scaffold
Cells
Nutrient Supply
Gas Supply
Bioreactor Housing
Closed Steril Circulation Loop
Roller Pump
Medium Reservoir
Perfusion
Superfusion
Mixed Operation Mode
Cell Lines
Primary Cells
Organotypic Functions
Hepatocellular Carcinoma Cell Lines
Liver Specific Genes
Article
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