Journal
/
Cancer Research
/
离心淘洗制备不同细胞周期的原发性急性淋巴细胞白血病细胞
JoVE Journal
Cancer Research
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Cancer Research
Preparation of Primary Acute Lymphoblastic Leukemia Cells in Different Cell Cycle Phases by Centrifugal Elutriation
Please note that all translations are automatically generated. Click here for the English version.

离心淘洗制备不同细胞周期的原发性急性淋巴细胞白血病细胞

DOI:
10.3791/56418-v

•

09:09 min

•

November 10, 2017

•

, , ,
1Department of Biochemistry and Molecular Biology,University of Arkansas for Medical Sciences, 2Department of Experimental Therapeutics,University of Texas MD Anderson Cancer Center

Chapters

  • 00:05Title
  • 00:41Assembling the Elutriation System
  • 02:55Priming the Elutriation System
  • 04:56Introduction of Cell Sample into the Elutriation System and Collection of Fractions
  • 06:56Results: Evaluation of Centrifugal Elutriation-based Sorting of Acute Lymphoblastic Leukemia Cells in Different Cell Cycle Phases
  • 08:21Conclusion

Summary

Automatic Translation

Automatic Translation

本议定书描述了使用离心淘洗分离原发性急性淋巴细胞白血病细胞到不同的细胞周期阶段。

Tags

Primary Acute Lymphoblastic LeukemiaCell Cycle PhasesCentrifugal ElutriationCell SeparationCancer TherapeuticsAnti-cancer CompoundsCell IsolationPhysical PropertiesCell CycleStrobe AssemblyRotorElutriation ChamberRotating Seal AssemblyFlow SystemBufferVariable Speed PumpSterilization5% Bleach

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

测定急性髓系白血病可测残留病和白血病干细胞的骨髓标本和过程综合协议

流式细胞仪估测原发性急性髓系白血病和患者源性移植中白血病干细胞的诊断和随访

复杂核型细胞周期捕获细胞的生成与分离

原发性白血病细胞代谢谱的评价

木尿造血干细胞和祖细胞和MLL-AF9驱动白血病线粒体反应氧物种的流细胞学分析

建立胃癌患者衍生异种移植模型和原细胞系

免疫荧光对非小细胞肺癌患者循环肿瘤细胞的半自动PD-L1特征化及枚举

细胞周期特异性测量βH2AX和细胞凋亡后热源毒性应力通过流细胞测定

原发性慢性淋巴细胞白血病细胞的细胞次细胞分段监测核/细胞质蛋白贩运

NKG2D 利甘德表面检测的两种流细胞学方法，以区分干细胞与急性骨髓性白血病中的散装亚群

Read Article