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Cancer Research
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遠心浄化によって異なった細胞周期の段階で主に急性リンパ性白血病細胞の調製
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Cancer Research
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JoVE Journal
Cancer Research
Preparation of Primary Acute Lymphoblastic Leukemia Cells in Different Cell Cycle Phases by Centrifugal Elutriation
Please note that all translations are automatically generated.
Click here for the English version.
遠心浄化によって異なった細胞周期の段階で主に急性リンパ性白血病細胞の調製
DOI:
10.3791/56418-v
•
09:09 min
•
November 10, 2017
•
Magdalena Delgado*
1
,
Anisha Kothari*
1
,
Walter N. Hittelman
,
Timothy C. Chambers
1
Department of Biochemistry and Molecular Biology
,
University of Arkansas for Medical Sciences
,
2
Department of Experimental Therapeutics
,
University of Texas MD Anderson Cancer Center
Chapters
00:05
Title
00:41
Assembling the Elutriation System
02:55
Priming the Elutriation System
04:56
Introduction of Cell Sample into the Elutriation System and Collection of Fractions
06:56
Results: Evaluation of Centrifugal Elutriation-based Sorting of Acute Lymphoblastic Leukemia Cells in Different Cell Cycle Phases
08:21
Conclusion
Summary
Automatic Translation
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Automatic Translation
このプロトコルでは、異なる細胞周期の段階に主な急性リンパ芽球性白血病細胞を分離する遠心浄化の使用について説明します。
Tags
Primary Acute Lymphoblastic Leukemia
Cell Cycle Phases
Centrifugal Elutriation
Cell Separation
Cancer Therapeutics
Anti-cancer Compounds
Cell Isolation
Physical Properties
Cell Cycle
Strobe Assembly
Rotor
Elutriation Chamber
Rotating Seal Assembly
Flow System
Buffer
Variable Speed Pump
Sterilization
5% Bleach
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