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增殖和静止人成纤维细胞的全基因组转录衰变率的测定
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Genetics
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JoVE Journal Genetics
Determining Genome-wide Transcript Decay Rates in Proliferating and Quiescent Human Fibroblasts
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增殖和静止人成纤维细胞的全基因组转录衰变率的测定

DOI:
10.3791/56423-v

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07:03 min

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January 02, 2018

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, ,
1Department of Molecular, Cell and Developmental Biology,University of California, Los Angeles, 2Department of Biological Chemistry,David Geffen School of Medicine, 3Molecular Biology Institute Interdepartmental Program,University of California, Los Angeles

Chapters

  • 00:05Title
  • 00:38ActD Time Course
  • 02:13Isolation of Total RNA from Phenol-guanidine Isothiocyanate Solution Lysate
  • 05:16Analysis of Transcript Abundance
  • 06:02Results: Clear Contrast in Transcript Decay Between Proliferating and Quiescent Fibroblasts
  • 06:28Conclusion

Summary

Automatic Translation

Automatic Translation

我们描述了一种生成增殖和静止的主要人真皮成纤维细胞的协议, 监测转录衰变率, 并识别差异腐烂的基因。

Tags

Genome-wideTranscriptDecay RatesProliferatingQuiescentFibroblastsMolecular BiologyGene ExpressionTranscript AbundanceActDPBSPhenol-guanidine IsothiocyanateSpike-inChloroform

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