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Metoder til undersøgelse ændringer i iboende Protein sammenlægning med alder i Caenorhabditis elegans
JoVE Journal
Biology
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JoVE Journal Biology
Methods to Study Changes in Inherent Protein Aggregation with Age in Caenorhabditis elegans
DOI:

11:57 min

November 26, 2017

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Chapters

  • 00:05Title
  • 00:53Liquid Culture for Treatment with RNAi Starting at L1
  • 02:40Collection of Young and Aged Worms Subjected to RNAi in Liquid Culture
  • 06:14Disruption of Animals
  • 06:58Isolation of SDS-insoluble Proteins for Mass Spectrometry
  • 10:03Results: Reduced IIS Prevents Age-dependent Protein Aggregation
  • 11:26Conclusion

Summary

Automatic Translation

Målet med den metode, der præsenteres her er at udforske protein sammenlægning under normal aldring i model organisme C. elegans. Protokollen repræsenterer et kraftfuldt værktøj til at studere meget tungtopløseligt store aggregater, der danner med alderen og at fastslå, hvordan ændringer i proteostasis påvirker protein sammenlægning.

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