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Chapters
- 00:05Title
- 00:53Liquid Culture for Treatment with RNAi Starting at L1
- 02:40Collection of Young and Aged Worms Subjected to RNAi in Liquid Culture
- 06:14Disruption of Animals
- 06:58Isolation of SDS-insoluble Proteins for Mass Spectrometry
- 10:03Results: Reduced IIS Prevents Age-dependent Protein Aggregation
- 11:26Conclusion
Målet med den metode, der præsenteres her er at udforske protein sammenlægning under normal aldring i model organisme C. elegans. Protokollen repræsenterer et kraftfuldt værktøj til at studere meget tungtopløseligt store aggregater, der danner med alderen og at fastslå, hvordan ændringer i proteostasis påvirker protein sammenlægning.
