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Chapters
- 00:05Title
- 00:53Liquid Culture for Treatment with RNAi Starting at L1
- 02:40Collection of Young and Aged Worms Subjected to RNAi in Liquid Culture
- 06:14Disruption of Animals
- 06:58Isolation of SDS-insoluble Proteins for Mass Spectrometry
- 10:03Results: Reduced IIS Prevents Age-dependent Protein Aggregation
- 11:26Conclusion
Målet med metoden som presenteres her er å utforske protein aggregering under normal aldring i modellen organismen C. elegans. Protokollen representerer et kraftig verktøy til å studere de svært uløselig store aggregatene som danner med alderen og bestemme hvordan endringer i proteostasis påvirker protein aggregering.
