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有丝分裂细胞同步与高分辨共聚焦显微镜联合应用研究细胞周期蛋白在有丝分裂过程中的作用
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Combining Mitotic Cell Synchronization and High Resolution Confocal Microscopy to Study the Role of Multifunctional Cell Cycle Proteins During Mitosis
Please note that all translations are automatically generated.
Click here for the English version.
有丝分裂细胞同步与高分辨共聚焦显微镜联合应用研究细胞周期蛋白在有丝分裂过程中的作用
DOI:
10.3791/56513-v
•
08:33 min
•
December 05, 2017
•
Mohammed A. Amin
,
Dileep Varma
1
Department of Cell and Molecular Biology, Feinberg School of Medicine
,
Northwestern University
Chapters
00:05
Title
00:52
Mitotic Cell Progression Fixed Cell Imaging
03:35
Mitotic Cell Progression Live Cell Imaging
05:31
Results: Representative Mitotic Progression in Control- and siRNA-depleted Cells
07:09
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
我们提出一个协议的双胸苷同步 HeLa 细胞后, 分析使用高分辨率共聚焦显微镜。这种方法是获得大量的细胞, 同步进行从 S 期到有丝分裂的关键, 使研究的有丝分裂作用的功能蛋白, 也具有相间的作用。
Tags
Cell Cycle
Mitosis
Cell Synchronization
Confocal Microscopy
Multifunctional Proteins
HeLa Cells
Thymidine Block
SiRNA
Immunofluorescence
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