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A Cell Free Assay to Study Chromatin Decondensation at the End of Mitosis
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A Cell Free Assay to Study Chromatin Decondensation at the End of Mitosis

A Cell Free Assay to Study Chromatin Decondensation at the End of Mitosis

DOI:
10.3791/53407-v

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11:04 min

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December 19, 2015

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, , ,
1Friedrich Miescher Laboratory,Max Planck Society, 2Nencki Institute of Experimental Biology,Polish Academy of Sciences

Chapters

  • 00:05Title
  • 00:48Protocol for Interphasic Xenopus Laevis Egg Extract
  • 05:38Protocol for In Vitro Reconstitution of Chromatin Decondensation
  • 08:56Results: Chromatin Decondensation is ATP and GTP Dependent
  • 09:48Conclusion

Summary

Automatic Translation

Automatic Translation

The molecular mechanisms of the decondensation of highly compacted mitotic chromatin are ill-defined. We present a cell-free assay based on mitotic chromatin clusters isolated from HeLa cells and Xenopus laevis egg extract that faithfully reconstitutes the decondensation process in vitro.

Tags

Keywords: Cell-free AssayChromatin DecondensationMitosisMitotic ExitNuclear EnvelopeX. Laevis Egg ExtractHeLa CellsMolecular EventsChromatin CondensationChromosome Structure

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