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Bioengineering
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用于内耳组织工程的 Decellularizing 小鼠耳蜗协议
JoVE Journal
Bioengineering
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JoVE Journal
Bioengineering
A Protocol for Decellularizing Mouse Cochleae for Inner Ear Tissue Engineering
Please note that all translations are automatically generated.
Click here for the English version.
用于内耳组织工程的 Decellularizing 小鼠耳蜗协议
DOI:
10.3791/56523-v
•
09:53 min
•
January 01, 2018
•
Christopher A. Neal
,
Jennifer G. Nelson-Brantley
,
Michael S. Detamore
,
Hinrich Staecker
,
Adam J. Mellott
1
Department of Otolaryngology
,
University of Kansas Medical Center
,
2
Stephenson School of Biomedical Engineering
,
University of Oklahoma
,
3
Department of Plastic Surgery
,
University of Kansas Medical Center
Chapters
00:05
Title
01:05
Temporal Bone Harvest and Cochlea Isolation
03:07
Cochlear Processing
05:11
Infusion of hWJCs into Decellularized Cochleae
06:58
Results: Cell Culture on Decellularized, Decalcified Mouse Cochleae
08:18
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
本协议的目的是演示一种有效的方法, decellularize 和 decalcify 小鼠耳蜗作为组织工程应用的支架使用。
Tags
Decellularization
Decalcification
Mouse Cochlea
Extracellular Matrix
Stem Cell Differentiation
Sensory Epithelium
Temporal Bone
Bony Labyrinth
Stapes
Oval Window
Round Window
Perilymph
1% SDS Solution
PBS
Antibiotic-antimycotic
Penicillin-streptomycin
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