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人线粒体 DNA 中核苷酸的同时定位与定量
JoVE Journal
Biology
This content is Free Access.
JoVE Journal
Biology
Simultaneous Mapping and Quantitation of Ribonucleotides in Human Mitochondrial DNA
Please note that all translations are automatically generated.
Click here for the English version.
人线粒体 DNA 中核苷酸的同时定位与定量
DOI:
10.3791/56551-v
•
12:35 min
•
November 14, 2017
•
Katrin Kreisel
,
Martin K.M. Engqvist
2
,
Anders R. Clausen
1
Department for Medical Biochemistry and Cell Biology
,
University of Gothenburg
,
2
Department of Biology and Biological Engineering
,
Chalmers University of Technology
Chapters
00:05
Title
00:55
HincII Treatment and Alkaline Hydrolysis
04:11
5′-End Phosphorylation
05:21
Single Strand DNA Ligation
06:35
Second Strand Synthesis
08:23
PCR Amplification, Library Analysis and Pooling
10:10
Results: Simultaneous Quantitation and Genome-wide Mapping of Ribonucleotides
11:39
Conclusion
Summary
Automatic Translation
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Automatic Translation
在这里, 我们描述一个方法可以同时定量和基因组范围内的地图核苷酸在高度完整的 dna 在核苷酸决议, 结合酶裂解的基因组 dna 与其碱性水解和随后5´端序.
Tags
Mitochondrial DNA
Ribonucleotides
DNA Integrity
DNA Replication
DNA Repair
HeLa Cells
HincII
Paramagnetic Beads
70% Ethanol
Potassium Hydroxide
Potassium Chloride
Sodium Acetate
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