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March 28, 2018
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Dieses Protokoll beschreibt eine Methode für die gleichzeitige Darstellung von Thalamocortical Axon Verzweigung und Synapse Bildung in organotypischen Kokulturen des Thalamus und Großhirnrinde. Individuelle Thalamocortical Axone und ihre präsynaptischen Terminals werden durch eine einzelne Zelle Elektroporation Technik mit DsRed und GFP-Tags Synaptophysin visualisiert.
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Cite this Article
Matsumoto, N., Yamamoto, N. Visualization of Thalamocortical Axon Branching and Synapse Formation in Organotypic Cocultures. J. Vis. Exp. (133), e56553, doi:10.3791/56553 (2018).
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