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脊髓 Cocultures 丘脑-皮质轴突分支和突触形成的可视化研究
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JoVE Journal Neuroscience
Visualization of Thalamocortical Axon Branching and Synapse Formation in Organotypic Cocultures
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脊髓 Cocultures 丘脑-皮质轴突分支和突触形成的可视化研究

DOI:
10.3791/56553-v

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06:16 min

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March 28, 2018

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,
1Department of Medical Neuroscience, Graduate School of Medical Sciences,Kanazawa University, 2Neuroscience Laboratories, Graduate School of Frontier Biosciences,Osaka University

Chapters

  • 00:04Title
  • 00:58Preparation of Cortical Slices
  • 02:24Procedure of Electroporation
  • 04:37Results: Thalamocortical Axons Coculture and Time-lapse Imaging
  • 05:38Conclusion

Summary

Automatic Translation

Automatic Translation

本协议描述了丘脑和大脑皮层脊髓 cocultures 中丘脑-皮质轴突分支和突触形成同时成像的方法。单个丘脑-皮质轴突及其突触前终端的可视化细胞电穿孔技术与 DsRed 和 GFP 标记突触素。

Tags

Keywords: ThalamocorticalAxon BranchingSynapse FormationOrganotypic CoculturesNeural Circuit FormationAxon GrowthSynapse FormationCerebral CortexPostnatal Day-two RatPrimary Visual CortexSomatosensory CortexCulture InsertThalamic BlockE15 EmbryoGlass CapillariesElectrode PipettePlasmid Injection

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