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JoVE Journal Neuroscience
脊髓 Cocultures 丘脑-皮质轴突分支和突触形成的可视化研究
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脊髓 Cocultures 丘脑-皮质轴突分支和突触形成的可视化研究

Article DOI: 10.3791/56553
March 28th, 2018 •
1,2, 2
1Department of Medical Neuroscience, Graduate School of Medical Sciences, Kanazawa University, 2Neuroscience Laboratories, Graduate School of Frontier Biosciences, Osaka University

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March 28th, 2018

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本协议描述了丘脑和大脑皮层脊髓 cocultures 中丘脑-皮质轴突分支和突触形成同时成像的方法。单个丘脑-皮质轴突及其突触前终端的可视化细胞电穿孔技术与 DsRed 和 GFP 标记突触素。

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