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JoVE Journal Neuroscience
Görselleştirme Thalamocortical Axon dallanma ve sinaps oluşumunda Organotypic Cocultures
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Görselleştirme Thalamocortical Axon dallanma ve sinaps oluşumunda Organotypic Cocultures

Article DOI: 10.3791/56553-v • 06:16 min • March 28th, 2018
March 28th, 2018 •
1,2, 2
1Department of Medical Neuroscience, Graduate School of Medical Sciences, Kanazawa University, 2Neuroscience Laboratories, Graduate School of Frontier Biosciences, Osaka University

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Bu iletişim kuralı thalamocortical akson dallanma ve synapse oluşumu organotypic cocultures, talamus ve serebral korteks eşzamanlı görüntüleme için bir yöntemi açıklar. Bireysel thalamocortical akson ve presynaptic onların terminalleri bir tek hücre çoğalmasıyla tekniği ile DsRed ve synaptophysin GFP öğesini tarafından görüntülenmiştir.

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Neuroscience sayı: 133 dilim kültür serebral korteks Talamus synaptogenesis aksonlar arborization kortikal gelişim hızlandırılmış görüntüleme
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