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JoVE Journal
Neuroscience

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JoVE Journal Neuroscience
Visualisering av Thalamocortical Axon forgrening og Synapse formasjon i Organotypic Cocultures
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Visualisering av Thalamocortical Axon forgrening og Synapse formasjon i Organotypic Cocultures

Article DOI: 10.3791/56553
March 28th, 2018 •
1,2, 2
1Department of Medical Neuroscience, Graduate School of Medical Sciences, Kanazawa University, 2Neuroscience Laboratories, Graduate School of Frontier Biosciences, Osaka University

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March 28th, 2018

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Denne protokollen beskriver en metode for samtidige bildebehandling thalamocortical axon forgrening og synapse formasjonen i organotypic cocultures av thalamus og hjernebarken. Personlige thalamocortical axons og deres presynaptic terminaler er visualisert ved en enkelt celle electroporation teknikk med DsRed og GFP-merket synaptophysin.

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