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Neuroscience

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JoVE Journal Neuroscience
Visualizzazione di Thalamocortical Axon ramificazione e Synapse formazione in Cocultures organotipiche
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Visualizzazione di Thalamocortical Axon ramificazione e Synapse formazione in Cocultures organotipiche

Article DOI: 10.3791/56553
March 28th, 2018 •
1,2, 2
1Department of Medical Neuroscience, Graduate School of Medical Sciences, Kanazawa University, 2Neuroscience Laboratories, Graduate School of Frontier Biosciences, Osaka University

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March 28th, 2018

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Questo protocollo descrive un metodo per l'acquisizione simultanea delle thalamocortical assone ramificazione e sinapsi formazione in organotipiche cocultures del talamo e corteccia cerebrale. Thalamocortical singoli assoni e loro terminali presinaptici sono visualizzati mediante una tecnica di elettroporazione di singola cellula con DsRed e lo synaptophysin GFP-etichettato.

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