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JoVE Journal Neuroscience
Visualisering av Thalamocortical Axon förgrening och synapsen bildande i Organotypic Cocultures
Ultrasound-Guided Microinjection into the Mouse Forebrain In Utero at E9.5
Journal (Neuroscience)
Ultrasound-Guided Microinjection into the Mouse Forebrain In Utero at E9.5
Getting Started with the LeaderFollowerByPhase Toolbox (Video) | JoVE
Journal (Neuroscience)
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Visualisering av Thalamocortical Axon förgrening och synapsen bildande i Organotypic Cocultures

Article DOI: 10.3791/56553-v • 06:16 min • March 28th, 2018
March 28th, 2018 •
1,2, 2
1Department of Medical Neuroscience, Graduate School of Medical Sciences, Kanazawa University, 2Neuroscience Laboratories, Graduate School of Frontier Biosciences, Osaka University

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Det här protokollet beskriver en metod för samtidig avbildning av thalamocortical axon förgrening och synapsen bildande i organotypic cocultures av thalamus och hjärnbarken. Enskilda thalamocortical axoner och deras presynaptiska terminaler visualiseras av en enda cell elektroporation teknik med DsRed och GFP-märkta synaptophysin.

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Neurovetenskap fråga 133 slice kultur hjärnbarken thalamus synaptogenes axonal arborization kortikala utveckling time-lapse imaging
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