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JoVE Journal Neuroscience
Visualización de axón talamocorticales ramificación y sinapsis formación en Organotypic Cocultures
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Visualización de axón talamocorticales ramificación y sinapsis formación en Organotypic Cocultures

Article DOI: 10.3791/56553-v • 06:16 min • March 28th, 2018
March 28th, 2018 •
1,2, 2
1Department of Medical Neuroscience, Graduate School of Medical Sciences, Kanazawa University, 2Neuroscience Laboratories, Graduate School of Frontier Biosciences, Osaka University

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Este protocolo describe un método para la proyección de imagen simultánea de talamocorticales axon ramas y sinapsis formación en organotypic cocultures del tálamo y corteza cerebral. Axones individuales talamocorticales y sus terminales presinápticos se visualizan mediante una técnica de electroporación unicelular con DsRed y synaptophysin tagged GFP.

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Neurociencia número 133 sector cultura corteza cerebral tálamo sinaptogénesis arborización axonal desarrollo cortical proyección de imagen de Time-lapse
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