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Journal / Biology / Objektivet-gratis Video mikroskopi for dynamisk…
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JoVE Journal Biology
Objektivet-gratis Video mikroskopi for dynamisk og kvantitativ analyse av tilhenger cellekultur
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Objektivet-gratis Video mikroskopi for dynamisk og kvantitativ analyse av tilhenger cellekultur

Article DOI: 10.3791/56580-v • 09:05 min • February 23rd, 2018
February 23rd, 2018 •
1, 1, 2, 2, 3,4, 3, 1, 1, 1, 5, 5, 4,6, 1
1CEA, LETI, DTBS, LISA, Université Grenoble Alpes, 2CEA, LETI, DTBS, LBAM, Université Grenoble Alpes, 3CEA, INSERM, BIG, Université Grenoble Alpes, 4CNRS, FR CNRS 3425, 5CNRS, UMR 144, Molecular Mechanisms of Intracellular Transport, PSL Research University, Institut Curie, 6TIMC-IMAG

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Objektivet-fri video mikroskopi kan vi overvåke cellekulturer direkte i inkubator. Her beskriver vi full protokollen for å hente og analysere en 2,7 dagen oppkjøpet av kultivert HeLa celler, fører til et datasett 2.2 x 10 spor6 målinger av personlige celle morfologi og 10584 celle syklus.

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Mobilnettet biologi problemet 132 linse-fri mikroskopi video cytometri tett cellekulturer kvantitativ mikroskopi
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