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Bioengineering
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毛细管阵列中多核酸的视觉检测
JoVE Journal
Bioengineering
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JoVE Journal
Bioengineering
Visual Detection of Multiple Nucleic Acids in a Capillary Array
Please note that all translations are automatically generated.
Click here for the English version.
毛细管阵列中多核酸的视觉检测
DOI:
10.3791/56597-v
•
08:56 min
•
November 15, 2017
•
Jianwei Chen*
1,2,3
,
Ning Shao*
1,2,3
,
Jiaying Hu*
4
,
Rong Li
,
Yuanshou Zhu
2,3
,
Dabing Zhang
5
,
Shujuan Guo
2,3
,
Junhou Hui
,
Peng Liu
,
Litao Yang
,
Sheng-Ce Tao
2,3
1
Key Laboratory of Systems Biomedicine (Ministry of Education), Shanghai Center for Systems Biomedicine
,
Shanghai Jiao Tong University
,
2
State Key Laboratory of Oncogenes and Related Genes
,
3
School of Biomedical Engineering
,
Shanghai Jiao Tong University
,
4
Collaborative Innovation Center for Biosafety of GMOs, National Center for the Molecular Characterization of Genetically Modified Organisms, School of Life Sciences and Biotechnology
,
Shanghai Jiao Tong University
,
5
Key Laboratory of Crop Marker-Assisted Breeding of Huaian Municipality
,
Jiangsu Collaborative Innovation Center of Regional Modern Agriculture and Environmental Protection
,
6
Department of Biomedical Engineering, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, School of Medicine
,
Tsinghua University
Chapters
00:05
Title
00:33
Fabrication of the Capillary Array-based Ready-to-use Cassette
03:55
Performance of LAMP Reaction in Capillary Array
05:07
Results Readout and Data Analysis
07:01
Results: The Specificity of the Cassette to Monitor GMOs
08:21
Conclusion
Summary
Automatic Translation
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Automatic Translation
该协议描述了一个小的, 现成的卡带的制作, 可用于视觉检测的多个核酸在一个单一的测试, 这是易于操作。在这种方法中, 用毛细管阵列对转基因生物的目标进行多重和高效的检测。
Tags
Nucleic Acid Detection
Capillary Array
Visual Detection
Multiplex
GMO Detection
PDMS
Capillary Cleaning
Piranha Solution
PDMS Curing
Hydrophobic Treatment
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