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मानव तंत्रिका प्रणेता कोशिकाओं में Neurodevelopmental Phenotypes का तेजी से पता लगाने (NPCs)
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1Department of Neuroscience and Cell Biology,Rutgers Robert Wood Johnson Medical School, 2Center for Advanced Biotechnology and Medicine, Department of Neuroscience and Cell Biology,Rutgers Robert Wood Johnson Medical School, 3The Child Health Institute of NJ, Department of Obstetrics, Gynecology, and Reproductive Services,Rutgers Robert Wood Johnson Medical School, 4The Child Health Institute of NJ, Department of Neuroscience and Cell Biology,Rutgers Robert Wood Johnson Medical School, 5Department of Genetics,Rutgers University
Chapters
- 00:04Title
- 01:08Collecting NPCs for Plating of Experimental Conditions
- 02:30Neural Precursor Cell DNA Synthesis Assay
- 03:47S Phase Entry Assay
- 04:39Neural Precursor Cell Number Assay
- 05:41Neural Precursor Cell Neurosphere Migration Assay
- 07:58Results: Use of Phase-contrast and Fluorescence Microscopy to Study the Proliferation, Migration, and Neurite Outgrowth of Neural Precursor Cells
- 09:51Conclusion
Neurodevelopmental प्रक्रियाओं जैसे प्रसार, प्रवासन, और neurite की वृद्धि अक्सर neuropsychiatric रोगों में परेशान हैं । इस प्रकार, हम तेजी से प्रोटोकॉल वर्तमान और reproducibly मानव iPSC-व्युत्पंन NPCs में इन neurodevelopmental प्रक्रियाओं का आकलन । इन प्रोटोकॉल भी प्रासंगिक विकास कारकों और चिकित्सकीय एनपीसी विकास पर प्रभाव के आकलन की अनुमति ।
