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Una identificación basada en redes neuronal desarrollo competentes o incompetentes adulto de ovocitos de ratón
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Una identificación basada en redes neuronal desarrollo competentes o incompetentes adulto de ovocitos de ratón

Article DOI: 10.3791/56668-v • 10:04 min • March 3rd, 2018
March 3rd, 2018 •
1, 1, 1, 1,2, 1,3, 4, 1, 1
1Laboratorio di Biologia dello Sviluppo, Dipartimento di Biologia e Biotecnologie "Lazzaro Spallanzani", University of Pavia, 2Institute of Biotechnology, University of Helsinki, 3Department of Reproductive Medicine, University of California San Diego, 4Dipartimento di Ingegneria Industriale e dell'Informazione, University of Pavia

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Aquí, presentamos un protocolo para la evaluación no invasiva de competencia desarrollo de oocito durante su maduración en vitro de la vesícula germinal a la etapa de metafase II. Este método combina la proyección de imagen de Time-lapse con partículas imagen velocimetry (PIV) y análisis de redes neuronales.

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Biología del desarrollo cuestión 133 ovocitos adulto transición de vesícula Germinal a la metafase II competencia desarrollo maduración In Vitro proyección de imagen de Time-lapse movimientos citoplasmáticos red neuronal Artificial de Feed-Forward
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