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Genetics
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昆虫细胞 (sf9) 外源基因在蛋白质功能检测中的瞬态表达
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Transient Expression of Foreign Genes in Insect Cells (sf9) for Protein Functional Assay
Please note that all translations are automatically generated.
Click here for the English version.
昆虫细胞 (sf9) 外源基因在蛋白质功能检测中的瞬态表达
DOI:
10.3791/56693-v
•
11:12 min
•
February 22, 2018
•
Ju-Chun Chang
,
Se Jin Lee
,
Jae Su Kim
,
Chung-Hsiung Wang
,
Yu-Shin Nai
1
Department of Biotechnology and Animal Science
,
National Ilan University
,
2
Department of Agricultural Biology, College of Agriculture Life Sciences
,
Chonbuk National University
,
3
Department of Entomology
,
National Taiwan University
Chapters
00:04
Title
00:52
Insect Cell Culture
01:53
Transient Protein Expression
05:00
Anti-apoptotic Activity Assay
07:04
Cell Viability Assay
08:11
Results: Transient Protein Expression Enables Analysis of Anti-apoptotic Activity
09:41
Conclusion
Summary
Automatic Translation
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Automatic Translation
本协议描述了一种热休克诱导蛋白表达系统 (pDHsp/V5-His/sf9 细胞系统), 可用于表达外来蛋白或评价潜在外来蛋白的抗凋亡活性及其截断的氨基酸昆虫细胞中的酸。
Tags
Transient Gene Expression
Insect Cells
Sf9
Protein Functional Assay
Anti-apoptotic Activity
Heat Shock Induction
Protein Expression
Cell Culture
Transfection
IAP3
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