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Forbigående udtryk af fremmede gener i insekt celler (sf9) for Protein funktionel analyse
JoVE Journal
Genetics
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JoVE Journal Genetics
Transient Expression of Foreign Genes in Insect Cells (sf9) for Protein Functional Assay
DOI:

11:12 min

February 22, 2018

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Chapters

  • 00:04Title
  • 00:52Insect Cell Culture
  • 01:53Transient Protein Expression
  • 05:00Anti-apoptotic Activity Assay
  • 07:04Cell Viability Assay
  • 08:11Results: Transient Protein Expression Enables Analysis of Anti-apoptotic Activity
  • 09:41Conclusion

Summary

Automatic Translation

Denne protokol beskriver en heat shock-induceret protein udtryk system (pDHsp/V5-hans/sf9 celle system), som kan bruges til enten at udtrykke fremmede proteiner eller evaluere det anti-apoptotiske aktivitet af potentielle udenlandske proteiner og deres afkortet amino syrer i insekt celler.

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