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Immunology and Infection
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荧光寿命成像显微镜观察细胞内圈套的贩运
JoVE Journal
Immunology and Infection
This content is Free Access.
JoVE Journal
Immunology and Infection
Visualizing Intracellular SNARE Trafficking by Fluorescence Lifetime Imaging Microscopy
Please note that all translations are automatically generated.
Click here for the English version.
荧光寿命成像显微镜观察细胞内圈套的贩运
DOI:
10.3791/56745-v
•
08:55 min
•
December 29, 2017
•
Daniëlle R. J. Verboogen
,
Maksim V. Baranov
,
Martin ter Beest
,
Geert van den Bogaart
1
Department of Tumor Immunology
,
Radboud University Medical Center
Chapters
00:05
Title
00:37
Preparation of the Microscopy Samples
01:49
Recording of the FLIM Data
03:34
Conversion of the Photon Recordings to FLIM Images
04:19
Fitting of Fluorescence Lifetime Histograms for Whole-cell FLIM Analysis
06:03
Results: Syntaxin 4 and VAMP3 Interaction in Live Cell Visualized by FRET-FLIM
07:43
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
该协议描述了一种新的方法, 允许定量可视化的复杂形成的圈套蛋白, 基于 rster 共振能量转移, 和荧光寿命成像显微镜。
Tags
SNARE
FLIM
Membrane Trafficking
HeLa Cells
Syntaxin-4
VAMP3
Syntaxin-3
MCitrine
MCherry
Fluorescence Lifetime Imaging Microscopy
Confocal Microscopy
Instrument Response Function
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