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JoVE Journal
Biochemistry

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高通量, 用定量斑点杂交分析法绝对测定组织层中选定蛋白质的含量 (QDB)
 
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高通量, 用定量斑点杂交分析法绝对测定组织层中选定蛋白质的含量 (QDB)

Article doi: 10.3791/56885
August 21st, 2018

Summary August 21st, 2018

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Click here for the English version.

在这里, 我们展示了定量斑点杂交分析 (QDB) 的详细过程, 通过确定目标蛋白的绝对含量, 帽肌动蛋白, gelsolin 样 (CAPG), 在三种不同的小鼠组织。我们展示了高通量, 方便, 定量应用免疫印迹技术的生物标志物验证在细胞和组织水平。

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