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Journal
/
Medicine
/
母乳促进 Enteroids 的生长: 一个
前
体细胞增殖模型
JoVE Journal
Medicine
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JoVE Journal
Medicine
Breast Milk Enhances Growth of Enteroids: An
Ex Vivo
Model of Cell Proliferation
Please note that all translations are automatically generated.
Click here for the English version.
母乳促进 Enteroids 的生长: 一个
前
体细胞增殖模型
DOI:
10.3791/56921-v
•
09:02 min
•
February 15, 2018
•
Wyatt E. Lanik
,
Lily Xu
,
Cliff J. Luke
,
Elise Z. Hu
,
Pranjal Agrawal
,
Victoria S. Liu
,
Rajesh Kumar
,
Alexa M. Bolock
,
Congrong Ma
,
Misty Good
1
Division of Newborn Medicine, Department of Pediatrics
,
Washington University School of Medicine
,
2
Washington University
,
3
Division of Newborn Medicine, Department of Pediatrics
,
University of Pittsburgh School of Medicine
Chapters
00:05
Title
00:57
Crypt Isolation and Establishment of Enteroids
04:08
Mouse Breast Milk Collection
05:03
Staining
07:35
Results: Breast Milk Increases Growth and Proliferation of Enteroids
08:10
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
该协议描述了如何建立从新生鼠或早肠的 enteroid 培养系统以及从小鼠收集牛奶的有效方法。
Tags
Breast Milk
Enteroids
Cell Proliferation
Ex Vivo
Gastrointestinal
Necrotizing Enterocolitis
Small Intestine
Crypt Isolation
Basement Membrane
Cell Disruption Media
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