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母乳促进 Enteroids 的生长: 一个前体细胞增殖模型
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JoVE Journal Medicine
Breast Milk Enhances Growth of Enteroids: An Ex Vivo Model of Cell Proliferation
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母乳促进 Enteroids 的生长: 一个前体细胞增殖模型

DOI:
10.3791/56921-v

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09:02 min

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February 15, 2018

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, , , , , , , , ,
1Division of Newborn Medicine, Department of Pediatrics,Washington University School of Medicine, 2Washington University, 3Division of Newborn Medicine, Department of Pediatrics,University of Pittsburgh School of Medicine

Chapters

  • 00:05Title
  • 00:57Crypt Isolation and Establishment of Enteroids
  • 04:08Mouse Breast Milk Collection
  • 05:03Staining
  • 07:35Results: Breast Milk Increases Growth and Proliferation of Enteroids
  • 08:10Conclusion

Summary

Automatic Translation

Automatic Translation

该协议描述了如何建立从新生鼠或早肠的 enteroid 培养系统以及从小鼠收集牛奶的有效方法。

Tags

Breast MilkEnteroidsCell ProliferationEx VivoGastrointestinalNecrotizing EnterocolitisSmall IntestineCrypt IsolationBasement MembraneCell Disruption Media

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