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含黄素线粒体脱氢酶同时测定超氧化物/过氧化氢和 NADH 生产

JoVE Journal
Biochemistry
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JoVE Journal Biochemistry

Simultaneous Measurement of Superoxide/Hydrogen Peroxide and NADH Production by Flavin-containing Mitochondrial Dehydrogenases

Please note that all translations are automatically generated. Click here for the English version.

含黄素线粒体脱氢酶同时测定超氧化物/过氧化氢和 NADH 生产

DOI:

10.3791/56975-v

•

08:57 min

•

February 24, 2018

•

Ryan J. Mailloux, Adrian Young, Marisa O’Brien, Robert Morris Gill

¹Department of Biochemistry,Memorial University of Newfoundland

Chapters

00:00Title
00:17Introduction
01:50Setting Up the Assay
05:44Results: Simultaneous tracking of changes in NADH and superoxide/hydrogen peroxide fluorescence
07:32Conclusion

Summary

Automatic Translation

线粒体含有几种黄素依赖的酶, 可以产生活性氧 (ROS)。由于不需要的副作用, 监测线粒体中单个部位的 ROS 释放具有挑战性。我们提出了一种简单, 廉价的方法直接评估的本地率的 ROS 释放使用纯化 flavoenzymes 和微板块荧光。

Tags

Mitochondrial Dehydrogenases Superoxide/hydrogen Peroxide NADH Production Flavin-containing Enzymes Pyruvate Dehydrogenase Alpha-ketoglutarate Dehydrogenase Microplate Fluorometry Reactive Oxygen Species Antioxidants Inhibitors Simultaneous Measurement

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