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Neuroscience
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从成年斑马鱼中制备新鲜视网膜切片用于
前
体成像实验
JoVE Journal
Neuroscience
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JoVE Journal
Neuroscience
Preparing Fresh Retinal Slices from Adult Zebrafish for
Ex Vivo
Imaging Experiments
Please note that all translations are automatically generated.
Click here for the English version.
从成年斑马鱼中制备新鲜视网膜切片用于
前
体成像实验
DOI:
10.3791/56977-v
•
11:01 min
•
May 09, 2018
•
Michelle M. Giarmarco
,
Whitney M. Cleghorn
,
James B. Hurley
2
,
Susan E. Brockerhoff
2
1
Department of Biochemistry
,
University of Washington
,
2
Department of Ophthalmology
,
University of Washington
Chapters
00:04
Title
00:44
Preparing Animals and Equipment
02:55
Dissecting the Retina
04:36
Flat-mounting the Retina
06:21
Slicing the Retina and Assembling the Imaging Ladder
08:39
Results: Visualizing Ca
2
-dynamics in Cone Photoreceptors
10:19
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
影像视网膜组织可以提供无法从传统生化方法收集的单细胞信息。该协议描述了从斑马鱼的视网膜切片的制备共焦成像。荧光基因编码的传感器或指示染料允许可视化许多生物学过程中的不同的视网膜细胞类型。
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Retinal Slices
Zebrafish
Ex Vivo Imaging
Fluorescent Proteins
Biosensors
Vision Research
Calcium Ions
Retinal Pigment Epithelium
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