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肠上皮细胞通透性的体外体内测定方法
JoVE Journal
Biology
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JoVE Journal Biology
In Vitro and In Vivo Approaches to Determine Intestinal Epithelial Cell Permeability
DOI:

10:22 min

October 19, 2018

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Chapters

  • 00:04Title
  • 01:18Plating and Maintenance of Caco-2bbe on Porous Polycarbonate Membranes
  • 02:53Use of Epithelial Meter for Measuring Transepithelial Electrical Resistance
  • 04:39Murine Model of Dextran Sulfate Sodium (DSS)-induced Colitis
  • 06:07Measuring the Epithelial Barrier Permeability in DSS-induced Colitis Mice
  • 08:01Results: Measure of Permeability of Intestinal Epithelial Cells
  • 09:29Conclusion

Summary

Automatic Translation

本文提出了两种方法来确定肠道屏障功能。在组织培养井中, 使用上皮表 (伏特/欧姆) 直接测量培养的上皮细胞的测定电阻。在小鼠中, 采用 FITC-葡聚糖灌胃法测定体内肠道通透性。

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