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肠上皮细胞通透性的体外及体内测定方法
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JoVE Journal Biology
In Vitro and In Vivo Approaches to Determine Intestinal Epithelial Cell Permeability
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肠上皮细胞通透性的体外及体内测定方法

DOI:
10.3791/57032-v

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10:22 min

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October 19, 2018

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, , , , , , , ,
1Jiangsu Key Laboratory of Neuropsychiatric Diseases and Cambridge-Suda (CAM-SU) Genome Resource Center,Soochow University, 2Department of Oncology,The First Affiliated Hospital of Soochow University

Chapters

  • 00:04Title
  • 01:18Plating and Maintenance of Caco-2bbe on Porous Polycarbonate Membranes
  • 02:53Use of Epithelial Meter for Measuring Transepithelial Electrical Resistance
  • 04:39Murine Model of Dextran Sulfate Sodium (DSS)-induced Colitis
  • 06:07Measuring the Epithelial Barrier Permeability in DSS-induced Colitis Mice
  • 08:01Results: Measure of Permeability of Intestinal Epithelial Cells
  • 09:29Conclusion

Summary

Automatic Translation

Automatic Translation

本文提出了两种方法来确定肠道屏障功能。在组织培养井中, 使用上皮表 (伏特/欧姆) 直接测量培养的上皮细胞的测定电阻。在小鼠中, 采用 FITC-葡聚糖灌胃法测定体内肠道通透性。

Tags

Intestinal Epithelial Cell PermeabilityIn VitroIn VivoInflammatory Bowel DiseaseGastrointestinal DiseasesBarrier IntegrityCaco-2bbe CellsTransepithelial Electrical Resistance (TER)Interferon Gamma

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