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Evaluación de la función de la vesícula extracelular durante la infección de la Malaria
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Evaluation of Extracellular Vesicle Function During Malaria Infection
DOI:

13:45 min

February 14, 2018

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Chapters

  • 00:04Title
  • 00:49Fluorescence Labeling of Extracellular Vesicles
  • 03:05Visualize Uptake of Extracellular Vesicles by Confocal Microscopy
  • 06:18Endothelial Cell Permeability
  • 07:36Determine Puromycin Sensitivity
  • 08:59Transduction of Endothelial Cells with Lentiviral Vector
  • 10:53Results: Use of Confocal Microscopy and Diffusion Capacity of Fluorochrome Dextran to Study the Interaction Between the Extracellular Vesicles and Endothelial Cells
  • 12:50Conclusion

Summary

Automatic Translation

En este trabajo, describimos protocolos para investigar el papel de las vesículas extracelulares (EVs) liberado por los eritrocitos Plasmodium falciparum infectados. En particular, nos centramos en las interacciones de EVs con las células endoteliales.

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