Journal
/
/
Evaluering af ekstracellulære vesikel funktion under Malaria infektion
JoVE Journal
Immunology and Infection
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Immunology and Infection
Evaluation of Extracellular Vesicle Function During Malaria Infection
DOI:

13:45 min

February 14, 2018

, , , , ,

Chapters

  • 00:04Title
  • 00:49Fluorescence Labeling of Extracellular Vesicles
  • 03:05Visualize Uptake of Extracellular Vesicles by Confocal Microscopy
  • 06:18Endothelial Cell Permeability
  • 07:36Determine Puromycin Sensitivity
  • 08:59Transduction of Endothelial Cells with Lentiviral Vector
  • 10:53Results: Use of Confocal Microscopy and Diffusion Capacity of Fluorochrome Dextran to Study the Interaction Between the Extracellular Vesicles and Endothelial Cells
  • 12:50Conclusion

Summary

Automatic Translation

I dette arbejde beskriver vi protokoller for at undersøge den rolle af ekstracellulære vesikler (EVs) udgivet af Plasmodium falciparum inficeret erytrocytter. Især fokuserer vi på samspillet mellem evt med endotelceller.

Related Videos

Read Article