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Genetics
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从
酿酒酵母
中组蛋白修饰的染色质沉淀 (芯片)
JoVE Journal
Genetics
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JoVE Journal
Genetics
Chromatin Immunoprecipitation (ChIP) of Histone Modifications from
Saccharomyces cerevisiae
Please note that all translations are automatically generated.
Click here for the English version.
从
酿酒酵母
中组蛋白修饰的染色质沉淀 (芯片)
DOI:
10.3791/57080-v
•
11:06 min
•
December 29, 2017
•
Meagan Jezek
,
Alison Jacques
,
Deepika Jaiswal
,
Erin M. Green
1
Department of Biological Sciences
,
University of Maryland
Chapters
00:05
Title
00:52
In Vivo
Crosslinking of Proteins to DNA
02:19
Making Yeast Lysates
04:08
Immunoprecipitation (IP) of Modified Histones
05:11
Washing IPs, Eluting Histone-DNA Complexes and Reversing Protein-DNA Crosslinks
07:24
Quantitative PCR (qPCR) to Detect Enriched Genomic Regions
08:46
Results: ChIP Reveals Histone Modification Status at ant Genomic Region
10:17
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
在这里, 我们描述了一个协议的染色质沉淀的修饰组蛋白从萌芽酵母
酿酒
酵母。Immunoprecipitated DNA 随后用于定量 PCR 研究组蛋白修饰修饰的丰度和局部化。
Tags
Chromatin Immunoprecipitation
ChIP
Histone Modifications
Saccharomyces Cerevisiae
Gene Expression Control
Environmental Conditions
Chromatin Binding Proteins
Epitope Tags
Antibodies
Yeast Cells
YPD Medium
Optical Density
Formaldehyde
Glycine
ChIP Lysis Buffer
PMSF
Protease Inhibitor
Glass Beads
Bead-beating
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