Journal
/
Genetics
/
En Optogenetic metode til at styre og analysere gen Expression mønstre i celle-til-celle interaktioner
JoVE Journal
Genetics
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Genetics
An Optogenetic Method to Control and Analyze Gene Expression Patterns in Cell-to-cell Interactions

En Optogenetic metode til at styre og analysere gen Expression mønstre i celle-til-celle interaktioner

DOI:
10.3791/57149-v

•

07:59 min

•

March 22, 2018

•

,
1Institute for Frontier Life and Medical Sciences,Kyoto University, 2Japan Science and Technology Agency,PRESTO, 3Institute for Integrated Cell-Material Sciences,Kyoto University, 4Graduate School of Medicine,Kyoto University, 5Graduate School of Biostudies,Kyoto University

Chapters

  • 00:04Title
  • 00:44Generation of Stable Cell Lines by the Tol2 System
  • 02:07Evaluation of Photo-sensitivity of Engineered Cells at the Population Level
  • 03:31Dynamic Sender-receiver Assay at the Population Level and at the Single-cell Level
  • 06:47Results: Analysis of Gene Expression Patterns in Cell-to-cell Interactions
  • 07:35Conclusion

Summary

Automatic Translation

Automatic Translation

Vi præsenterer her, en protokol for at analysere celle til celle overførsel af oscillerende oplysninger optogenetic kontrol og live overvågning af genekspression. Denne fremgangsmåde giver en enestående platform for at teste en funktionel betydning af dynamiske gen expression programmer i flercellede systemer.

Tags

OptogeneticsCell-to-cell InteractionsNotch Signaling PathwayGene Expression DynamicsTol2-based Optogenetic ModulesC2C12 CellsFluorescent Protein SelectionLight-induced ConditionsProgrammable Light Illumination

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

Genetisk Manipulation af afgroedesygdomme<em> Ustilago maydis</em> At studere Fungal Biology og Plant Microbe Interactions

Parallel Måling af døgnrytmen ur genekspression og hormon sekretion i Human primære cellekulturer

Optogenetic Tilfældig mutagenese ved anvendelse histon-miniSOG i<em> C. elegans</em

Single-celle genekspression Brug Multiplex RT-qPCR at Karakterisere Meget forskellige sjældne Lymfoide populationer

Single-celle Gene Expression Profiling Brug FACS og qPCR med interne standarder

Studie af Cyklusreguleret Gen Expression af To Komplementære Celle Synkronisering Protokoller

Forberedelse af prøver og analyser af RNASeq-baseret genekspression Data fra zebrafisk

Måling af genekspression i bombarderede Byg Aleurone lag med øget gennemløb

En metode til at studere C924T polymorfi af tromboxan A2 Receptor genet

En Ecdysone Receptor-baserede ental gen Switch til bevidst udtryk for transgen med robusthed, reversibilitet og ubetydelig Leakiness

Read Article