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Journal
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Immunology and Infection
/
尺寸事项:
新生隐球菌
中胶囊直径的测定
JoVE Journal
Immunology and Infection
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JoVE Journal
Immunology and Infection
Size Matters: Measurement of Capsule Diameter in
Cryptococcus neoformans
Please note that all translations are automatically generated.
Click here for the English version.
尺寸事项:
新生隐球菌
中胶囊直径的测定
DOI:
10.3791/57171-v
•
08:24 min
•
February 27, 2018
•
Tiffany Guess
,
Hoyin Lai
,
Serenah E. Smith
,
Linda Sircy
,
Kirsten Cunningham
,
David E. Nelson*
1
,
Erin E. McClelland*
1
1
Department of Biology
,
Middle Tennessee State University
,
2
DRVision Technologies
Chapters
00:04
Title
00:50
Capsule Induction and Yeast Cell Staining
03:14
Image Acquisition
04:27
Capsule Measurement
06:33
Results: Representative Capsule Measurement
07:58
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
多糖胶囊是
新生隐球菌
的主要毒力因子, 其大小与菌株的毒力密切相关。胶囊直径测量用于表型检测和测量疗效。本文提出了一种标准的胶囊诱导方法, 并比较了两种染色法和测量直径法。
Tags
Cryptococcus Neoformans
Capsule Diameter
Capsule Staining
India Ink
Calcofluor-white
Alexa Fluor 488
Capsule Measurement
Virulence
Automated Analysis
Article
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