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Paraffin inbäddning och tunn snittning av mikrobiell kolonin biofilmer för Mikroskopisk analys
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Paraffin Embedding and Thin Sectioning of Microbial Colony Biofilms for Microscopic Analysis
DOI:

08:52 min

March 23, 2018

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Chapters

  • 00:04Title
  • 00:49Growth of Pseudomonas aeruginosa Colony Biofilms and Overlaying Colonies with Agar
  • 02:33Fixation and Sample Processing
  • 03:02Sample Embedding and Sectioning
  • 05:23Heat Fixing, Rehydration, and Mounting
  • 06:48Results: Microscopic Analysis of Microbial Colony Biofilms
  • 08:01Conclusion

Summary

Automatic Translation

Vi beskriver fixering, paraffin inbäddning och tunn snittningen tekniker för mikrobiell kolonin biofilmer. I beredda prover, kan biofilm underkonstruktion och reporter uttrycksmönster visualiseras genom mikroskopi.

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