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Analysis of the Lipid Composition of Mycobacteria by Thin Layer Chromatography
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Immunology and Infection
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JoVE Journal Immunology and Infection
Analysis of the Lipid Composition of Mycobacteria by Thin Layer Chromatography

Analysis of the Lipid Composition of Mycobacteria by Thin Layer Chromatography

DOI:
10.3791/62368-v

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07:42 min

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April 16, 2021

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, ,
1Departament de Genètica i de Microbiologia, Facultat de Biociències,Universitat Autònoma de Barcelona

Chapters

  • 00:05Introduction
  • 00:33Extraction of The Total Non-Covalent-Linked Lipids from Mycobacteria
  • 02:13Mycolic Acid Extraction by Acid Methanolysis
  • 03:26Thin Layer Chromatography (TLC) Analysis
  • 04:57Results: TLC Analysis of the Lipid Composition in Different Species of Mycobacteria
  • 07:15Conclusion

Summary

Automatic Translation

Automatic Translation

A protocol is presented to extract the total lipid content of the cell wall of a wide range of mycobacteria. Moreover, extraction and analytical protocols of the different types of mycolic acids are shown. A thin-layer chromatographic protocol to monitor these mycobacterial compounds is also provided.

Tags

Keywords: MycobacteriaLipidsThin Layer ChromatographyExtractionChloroformMethanolMycolic AcidN-hexaneTLC

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